TEER Scanner
The transwell diffusion chamber is a widely employed in vitro model for studying biological barrier tissues. It involves cultivating a cell layer on a porous membrane within a transwell insert. The quality of this cell layer's seal can be assessed by measuring the Transepithelial Electrical Resistance (TEER) between the apical and basolateral compartments, with a cell line-dependent characteristic TEER value indicating a well-sealed barrier. The TEER Scanner system automates the repetitive measurements of transwell cultures in multi-well plates.
Our scanner is designed to streamline TEER measurements: it moves and positions either the MERSSTX01 electrode used in the Millicell ERS-2 voltohmmeter, or a similar chopstick electrode to a series of cultures. Each culture is precisely positioned within a multi-well plate with our positioning device. This approach significantly reduces measurement time, minimizing potential pH and temperature drift in the medium. Moreover, it enhances reproducibility by positioning the electrodes with a remarkable 0.1 mm accuracy, freeing laboratory staff from monotonous, manual tasks.
Daily TEER values collected from transwell MDCK cultures.
